Catalog Number - 68522-10P-2

68522-10P-2 Cogent RP PFP™ HPLC Column has been a premium value trusted by HPLC laboratories for many years, delivering reliable performance and enhanced selectivity for complex separations. This column is fully double end‑capped and bonded via a four‑carbon linker to a benzene ring substituted with five fluorine atoms, using ultra‑high purity silica.

The column provides multiple retention mechanisms—including π‑π interactions, dipole interactions, and hydrogen bonding—making it particularly effective for halogenated, aromatic, and polar analytes. It is also suitable as an alternate column for USP methods requiring L43 classification.

With a 5 µm particle size, this column offers lower backpressure compared to smaller particle columns, providing improved robustness and compatibility while maintaining strong chromatographic efficiency.

100 mm Length – Balanced Resolution and Speed

The 100 mm length offers an ideal balance between resolution and run time, making it well suited for routine analytical workflows and method development.

• Improved resolution compared to 50 mm columns

• Faster run times than longer column formats

• Increased peak capacity for moderately complex samples

✅ Recommended for LC‑MS workflows and routine analytical separations

Flow Rate & System Requirements (2.1 mm ID, 5 µm)

⚠️ Designed for low‑flow applications requiring stable pump performance

• Typical flow rate range: ~0.2 – 0.4 mL/min

• Lower backpressure than 3 µm columns

• Compatible with LC‑MS and low‑flow HPLC systems

• Increased method robustness

✅ Important: Standard HPLC systems must be capable of reliable, stable low flow rates

✅ Best Practice: Filter samples using a 0.2 µm syringe filter prior to injection

Why Choose PFP (F5) Columns for Selective Separations

Enhanced Retention for Halogenated Compounds

• Strong interaction with fluorinated and halogenated analytes

• Improved selectivity vs C18 columns

Multiple Interaction Mechanisms

• π‑π interactions for aromatic compounds

• Dipole and hydrogen bonding interactions

• Greater flexibility for method development

100 Å Pore Structure

• Suitable for small to moderately sized molecules

• Broader application range and versatility

Cogent RP PFP™ Column Advantages

Fully End‑Capped Surface

• Minimizes silanol activity

• Improves peak symmetry

Robust Performance (5 µm Particles)

• Lower backpressure vs smaller particle sizes

• Reliable and consistent operation

High Purity Silica

• Reproducible chromatographic performance

• Long column lifetime

Typical Applications

• Halogenated compound analysis

• Aromatic compound separations

• Polar analyte retention

• LC‑MS workflows

• Method development and optimization

• USP L43 methods

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Designed for LC‑MS and low‑flow HPLC systems (2.1 mm ID)

• Lower backpressure compared to smaller particle columns

• Requires accurate low‑flow pump capability

• Use 0.2 µm filtration prior to injection